Essentials of Single-Cell Analysis: Concepts, Applications and Future Prospects
Springer | Engineering | February 22, 2016 | ISBN-10: 3662491168 | 414 pages | pdf | 11.86 mb
Editors: Tseng, Fan-Gang, Santra, Tuhin Subhra (Eds.)
Presents various stimuli introduction methods, including electroporation, microinjection, mechanoporation, optoporation or photoporation in detail
Covers different fluidic systems and their integration with sensor technology, optical and hydrodynamic stretcher etc.
Demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems
This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations.
This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis.
Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan.
Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
Number of Illustrations and Tables
15 illus., 127 in colour
Topics
Biomedical Engineering
Microengineering
Bioinformatics
Physics of the Cell
Cell Biology
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